Description
Product Usage Information
Western Blotting: 1:1000
Immunoprecipitation: 1:200
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting, Immunoprecipitation
Specificity / Sensitivity
USP20 (F2T8S) Rabbit Monoclonal Antibody recognizes endogenous levels of total USP20 protein. This antibody detects a 28 kDa protein of unknown identity in some cell lines.
Species Reactivity: Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human USP20 protein.
Background
Ubiquitin-specific-processing protease 20 (USP20), also known as ubiquitin carboxyl-terminal hydrolase 20, is a deubiquitinating enzyme (DUB) and belongs to the USP subclass of DUB (1). Studies show that, after feeding, increased circulating insulin and glucose activate mTORC1, which phosphorylates USP20 at Ser132 and Ser134. The phosphorylated USP20, in turn, is recruited to the HMG-CoA reductase (HMGCR) complex. HMGCR is the rate-limiting enzyme in the cholesterol biosynthesis pathway. HMGCR stabilization through deubiquitination by USP20 in the feeding state, therefore, leads to elevated cholesterol biosynthesis in the liver (2). Furthermore, USP20 was identified as the DUB stabilizing the labile transcription factor SNAI2/SLUG to promote breast cancer metastasis (3).
Alternate Names
Deubiquitinating enzyme 20; hVDU2; KIAA1003; LSFR3A; pVHL-interacting deubiquitinating enzyme 2; Ubiquitin carboxyl-terminal hydrolase 20; ubiquitin specific peptidase 20; ubiquitin specific protease 20; Ubiquitin thioesterase 20; ubiquitin thiolesterase 20; Ubiquitin-specific-processing protease 20; UBP20; USP20; VDU2; VHL-interacting deubiquitinating enzyme 2
