Description
Product Usage Information
Western Blotting: 1:1000
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting
Specificity / Sensitivity
Complement C4-A/C4-B (E6J7W) Rabbit Monoclonal Antibody recognizes endogenous levels of total C4-A/C4-B protein. The predicted epitope of this antibody is the N-terminus of the β-subunit of the C4-A/C4-B protein.
Species Reactivity: Human, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro148 of human C4-A/C4-B protein.
Background
Complement is a collection of soluble proteins secreted in the blood and other body fluids (1). As part of the innate immune system, complement proteins are involved in the clearance of pathogens and damaged cells in a process called opsonization, which results in the coating of a pathogen with antibodies and/or complement proteins to facilitate phagocytosis of debris or foreign pathogens. Complements are activated by a cascade of cleavage reactions, triggered initially by pattern recognition receptor-mediated detection of pathogens/debris. The cascade of cleavage-mediated products activate three distinct effector pathways, including inflammation, phagocytosis, and membrane attack that represent a coordinated defense of the host organism. Several complement proteins are encoded in the mammalian genome, designated by the capital letter C followed by a number, in order by their discovery. Complement C4-A and C4-B, encoded by the and genes, respectively, are the non-enzymatic components of C3 and C5 convertases and thus essential for the propagation of the classical complement pathway. The / genes generate C4-A/C4-B polypeptide proteins, composed of an alpha, beta, and gamma-chain subunits linked by disulfide bonds. The polypeptide protein can be further cleaved to generate a cascade of fragments to initiate the innate immune response.  In addition to the innate immune response, several components of the complement system, including C4-A, have been implicated in brain development, as well as neurodevelopmental and neurodegenerative diseases. The complement system plays a role in microglial-dependent synapse pruning of excess synapses during development, particularly in the visual system (2). Additionally, complement-mediated synaptic pruning may also contribute to psychiatric diseases such as schizophrenia (3,4).
Alternate Names
acidic C4; Acidic complement C4; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 2; C4; C4A; C4a anaphylatoxin; C4A2; C4A3; C4A4; C4A6; C4AD; C4b-A; C4d-A; C4S; CO4; CO4A; Complement C4 beta chain; Complement C4 gamma chain; Complement C4-A; Complement C4-A alpha chain; complement C4A (Rodgers blood group); complement component 4A (Rodgers blood group); CPAMD2; MGC164979; MHC class III region complement; RG; Rodgers form of C4
