Description
Product Usage Information
Immunohistochemistry (Paraffin): 1:50 - 1:200
Immunofluorescence (Immunocytochemistry): 1:800 - 1:3200
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Immunohistochemistry (Paraffin), Immunofluorescence (Immunocytochemistry)
Specificity / Sensitivity
MITF (D5) Mouse Monoclonal Antibody recognizes endogenous levels of total MITF protein.
Species Reactivity: Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a His-tagged fusion protein corresponding to an amino-terminal fragment of human MITF protein (7).
Background
Microphthalmia-associated transcription factor (MITF) is a basic helix-loop-helix leucine zipper transcription factor that is most widely known for its roles in melanocyte, ophthalmic, and osteoclast development (1-3). In humans, MITF can function as a melanoma oncogene (4) and mutations in the corresponding gene are associated with Waardenburg syndrome type 2, an auditory-pigmentary syndrome characterized by developmental defects in cells derived from neural crest (5). At least 12 isoforms of MITF have been identified, which exhibit differential patterns of expression among cell and tissue types (6).
Alternate Names
BHLHE32; Class E basic helix-loop-helix protein 32; CMM8; COMMAD; homolog of mouse microphthalmia; melanocyte inducing transcription factor; melanogenesis associated transcription factor; MI; microphtalmia-associated transcription factor; Microphthalmia-associated transcription factor; microphthalmia-associated transcription factor-M transcript; MITF; WS2; WS2A
