Description
Product Usage Information
Western Blotting: 1:1000
Immunoprecipitation: 1:50
Immunofluorescence (Immunocytochemistry): 1:50 - 1:200
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Immunocytochemistry)
Specificity / Sensitivity
RBM4 (E1Y2G) Rabbit Monoclonal Antibody recognizes endogenous levels of total RBM4 protein. This antibody does not cross-react with RBM4B protein.
Species Reactivity: Human, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro317 of human RBM4 protein.
Background
RBM4 functions as an RNA-binding protein and precursor mRNA splicing factor that antagonizes activity of PTBP1-mediated splicing (1-4). Upon phosphorylation at Ser309, RBM4 accumulates in stress granules and suppresses cap-dependent translation through eIF4A (3). Exon skipping of MEF2C by RBM4 has been shown to play a key role in the differentiation of brown adipocytes (5). The splicing activities of RBM4 seem to suppress tumorigenesis in the context of multiple cancer types (5-8).
Alternate Names
DKFZp547K0918; hLark; LARK; Lark homolog; MGC75138; RBM4; RBM4A; RNA binding motif protein 4; RNA-binding motif protein 4; RNA-binding motif protein 4a; RNA-binding protein 4; transcriptional coactivator CoAZ; ZCCHC21; ZCRB3A; zinc finger CCHC-type and RNA binding motif 3A
