Description
Product Usage Information
Western Blotting: 1:1000
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting
Specificity / Sensitivity
PFAS Antibody recognizes endogenous levels of total PFAS protein.
Species Reactivity: Human, Mouse, Rat, Monkey
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro413 of human PFAS protein. Antibodies are purified by peptide affinity chromatography.
Background
Phosphoribosylformylglycinamidine synthase (PFAS) is a metabolic enzyme (deamidase) associated with a protein complex known as the "purinosome," which is involved in purine synthesis (1). Research studies have shown that activation of the MAPK/ERK pathway leads to downstream activation of PFAS. Specifically, it was shown that PFAS is a substrate of ERK2, which phosphorylates PFAS at Thr619; activation of PFAS results in the generation of purine intermediates required for cell and tumor growth (2). It has also been reported that both PFAS and viral PFAS-like proteins can activate RIG-I pattern recognition receptors, implicating PFAS-mediated deamidation in the innate immune response to viral infection (3).
Alternate Names
FGAM synthase; FGAMS; FGAR amidotransferase; FGAR-AT; FGARAT; Formylglycinamide ribonucleotide amidotransferase; Formylglycinamide ribotide amidotransferase; Formylglycinamide ribotide synthetase; GATD8; IKZF1/PFAS fusion; KIAA0361; PFAS; Phosphoribosylformylglycinamidine synthase; PUR4; PURL
