Description
Product Usage Information
Western Blotting: 1:1000
Simple Western™: 1:10 - 1:50
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting
Specificity / Sensitivity
PRRX1 (F3D7A) Rabbit Monoclonal Antibody recognizes endogenous levels of total PRRX1 protein.
Species Reactivity: Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human PRRX1 protein.
Background
Paired-related homeobox protein 1 (PRX1 or PRRX1) is a member of the paired-type family of homeobox transcription factors. In early development, the protein is expressed in mesenchymal cells in the craniomaxillofacial, limb, and pulmonary vascular systems to ensure normal tissue formation (1-3). PRRX1 is expressed in various types of cancer, including colon, stomach, lung, esophageal, and breast cancers. It functions as a master transcription factor of stromal fibroblasts for myofibroblastic lineage progression to promote tumorigenicity and metastasis (4). In glioma, PRRX1 promotes stemness and angiogenesis via activating the TGF-β/SMAD pathway and upregulating proangiogenic factors (5). PRRX1 interacts with FoxM1 to promote the DNA Damage Response (DDR) in pancreatic cancer cells (6). The protein also drives tamoxifen therapy resistance through inducing epithelial-mesenchymal transition (EMT) in breast cancer cells (7). PRRX1 exists as two isoforms, PRRX1a and PRRX1b. Isoform PRRX1a stimulates metastatic outgrowth, tumor differentiation, and mesenchymal-epithelial transition (MET), while isoform PRRX1b promotes tumor invasion, dedifferentiation, and EMT (8).
Alternate Names
AGOTC; Homeobox protein PHOX1; paired mesoderm homeo box 1; paired mesoderm homeobox 1 isoform pmx-1b; Paired mesoderm homeobox protein 1; paired related homeobox 1; Paired-related homeobox protein 1; PHOX1; PMX1; PRRX1; PRX-1; PRX1
