Description
Product Usage Information
Western Blotting: 1:1000
Immunofluorescence (Frozen): 1:200 - 1:800
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting, Immunofluorescence (Frozen)
Specificity / Sensitivity
CD200 (F6V8L) Rabbit Monoclonal Antibody recognizes endogenous levels of total CD200 protein.
Species Reactivity: Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of mouse CD200 protein.
Background
CD200 (OX2) and CD200R (OX2R) are membrane glycoprotein members of the Ig superfamily (1-3). CD200 is expressed by a range of cells, including neurons, epithelial cells, endothelial cells, fibroblasts, and lymphoid cells, while its receptor, CD200R, is found on myeloid and T cells (1-5). Interaction between CD200 and CD200R downregulates macrophage function and plays a role in immunosuppression and regulation of anti-tumor immune responses (3-7).
Alternate Names
antigen identified by monoclonal antibody MRC OX-2; Cd200; CD200 antigen; Mox; Mox2; MRC OX-2 antigen; OX-2 membrane glycoprotein; OX2; OX2G
