Description
Product Usage Information
Immunofluorescence (Frozen): 1:100
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Immunofluorescence (Frozen)
Specificity / Sensitivity
GAD1 (A9A5X) Rabbit Monoclonal Antibody recognizes endogenous levels of total GAD1 protein. This antibody cross-reacts with serum albumin.
Species Reactivity: Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala15 of human GAD1 protein.
Background
The enzyme glutamate decarboxylase (GAD) is responsible for the synthesis of the essential neurotransmitter gamma-aminobutyric acid (GABA) from L-glutamic acid (1). GAD1 (GAD67) and GAD2 (GAD65) are expressed in nervous and endocrine systems (2) and are thought to be involved in synaptic transmission (3) and insulin secretion (4), respectively. Autoantibodies against GAD2 may serve as markers for type I diabetes (5). Many individuals suffering from an adult onset disorder known as Stiff Person Syndrome (SPS) also express autoantibodies to GAD2 (6). GAD1 and GAD2 are encoded by separate genes and are coexpressed in most of the GABA-containing neurons (1, 7).
Alternate Names
67 kDa glutamic acid decarboxylase; CPSQ1; DCE1; FLJ45882; GAD; GAD-67; GAD1; GAD67; Glutamate decarboxylase 1; glutamate decarboxylase 1 (brain, 67kDa); Glutamate decarboxylase 67 kDa isoform; SCP
