Description
Product Usage Information
Western Blotting: 1:1000
Immunoprecipitation: 1:50
Flow Cytometry (Fixed/Permeabilized): 1:100 - 1:400
Flow Cytometry (Live): 1:100 - 1:400
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting, Immunoprecipitation, Flow Cytometry (Fixed/Permeabilized), Flow Cytometry (Live)
Specificity / Sensitivity
CD58 (F7R6O) Rabbit Monoclonal Antibody recognizes endogenous levels of total CD58 protein.
Species Reactivity: Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the extracellular domain of human CD58 protein.
Background
CD58, also known as lymphocyte function-associated antigen 3 (LFA-3), is a ubiquitously expressed glycoprotein anchored to the plasma membrane through a transmembrane domain or a GPI linkage (1). Research studies have demonstrated that CD58 plays a critical role in immune synapse formation by functioning as a co-stimulatory adhesion molecule upon engagement of the CD2 antigen on the surface of T cells and NK cells. As such, the CD58-CD2 axis facilitates the execution of immune effector cell functions, including activation, proliferation, and cytotoxicity (2-4). In the context of cancer, recent studies have pointed to the CD58-CD2 axis as a stimulatory immune checkpoint, and lesions to this axis compromise anti-tumor immune responses (5,6).
Alternate Names
Ag3; CD58; CD58 antigen, (lymphocyte function-associated antigen 3); CD58 molecule; LFA-3; LFA3; Lymphocyte function-associated antigen 3; Surface glycoprotein LFA-3
