Description
Product Usage Information
For more information about the RBP-eCLIP service please visit Eclipsebio .
Western Blotting: 1:1000
Immunoprecipitation: 1:50
eCLIP: 1:200
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody .
Protocol
Available protocols: Western Blotting, Immunoprecipitation
Specificity / Sensitivity
NKRF (E9G1T) Rabbit Monoclonal Antibody recognizes endogenous levels of total NKRF protein.
Species Reactivity: Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro75 of human NKRF protein.
Background
NF-κB Repressing Factor (NKRF) is a constitutively expressed transcription factor that binds to the negative regulatory element within the promotor region of many genes that are transactivated by NF-κB (1). Indeed, research studies have found NKRF to repress transcription of IL-8/CXCL8, iNOS, CXCL10, and IFN-γ (2-4). In addition to regulating the expression of pro-inflammatory factors, NKRF has been shown to play a role in cell survival in the context of proteotoxic stress, through the regulation of rRNA processing and ribosome biogenesis (5).
Alternate Names
ITBA4; NF-kappa-B-repressing factor; NFKB repressing factor; NFkB-repressing factor; NKRF; NRF; Protein ITBA4; Transcription factor NRF
