Description
Product Usage Information
Flow Cytometry (Fixed/Permeabilized): 1:100 - 1:400
Flow Cytometry (Live): 1:100 - 1:400
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Flow Cytometry (Fixed/Permeabilized), Flow Cytometry (Live)
Specificity / Sensitivity
Siglec-7 (F6K8J) Rabbit Monoclonal Antibody recognizes endogenous levels of total Siglec-7 protein.
Species Reactivity: Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human Siglec-7 protein.
Background
Sialic acid-binding Ig-like lectin 7 (Siglec-7), also known as AIRM1, is a member of the CD33-related Siglec family, a subset of the immunoglobulin superfamily of cell surface proteins (1-3). Siglec-7 is found in human immune cells, such as natural killer (NK) cells, monocytes, and neutrophils (1,2). Siglec-7 acts as an immune inhibitory receptor via its intracellular tyrosine-based inhibitory motif (ITIM) (1-5). Downregulation of cell surface Siglec-7 is an early marker of NK cell dysfunction, which can be associated with chronic viral infection or impaired anti-tumor activity (4).
Alternate Names
Adhesion inhibitory receptor molecule 1; adhesion inhibitory receptor molecule 1, siglec-7; AIRM-1; AIRM1; CD328; CDw328; D-siglec; p75; p75/AIRM1; QA79; QA79 membrane protein; sialic acid binding Ig like lectin 7; sialic acid binding Ig-like lectin 7; sialic acid binding immunoglobulin-like lectin 7; Sialic acid-binding Ig-like lectin 7; SIGL7; Siglec-7; SIGLEC19P; SIGLEC7; SIGLECP2
