Description
Product Usage Information
Western Blotting: 1:1000
Immunoprecipitation: 1:200
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting, Immunoprecipitation
Specificity / Sensitivity
A1/Bfl-1 (E4P2I) Rabbit Monoclonal Antibody recognizes endogenous levels of total A1/Bfl-1 protein. A strong band of unknown origin is detected at 28 kDa in some cell lines.
Species Reactivity: Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of mouse A1/Bfl-1 protein.
Background
The Bcl-2-related protein A1 (Bfl-1, BCL2A1) is an anti-apoptotic member of the Bcl-2 family originally cloned from mouse bone marrow as a granulocyte macrophage-colony stimulating factor (GM-CSF)-inducible gene (1). Expression of A1/Bfl-1 is primarily restricted to hematopoietic cells, although it has been detected in some non-hematopoietic tissues including lung and in endothelial cells (1,2). A1/Bfl-1 protein is rapidly induced by NF-κB and is elevated in response to a variety of factors that stimulate this pathway, including TNF-α and IL-1β, CD40, phorbol ester, and LPS (2-4). As with other Bcl-2 family proteins, A1/Bfl-1 functions by binding and antagonizing pro-apoptotic members of the family (Bid, Bim), which inhibits release of mitochondrial cytochrome c (5). In contrast, research studies indicate that the enzyme calpain cleaves A1/Bfl-1 at specific sites within the amino-terminal region, creating pro-apoptotic, carboxy-terminal fragments that promote mitochondrial release of cytochrome c and apoptosis (6). Studies suggest a possible therapeutic strategy of targeting apoptosis through use of the specific A1/Bfl-1 cleavage fragments (7).
Alternate Names
A1-A; B cell leukemia/lymphoma 2 related protein A1a; B2LA1; BB218357; Bcl-2-related protein A1; Bcl2; Bcl2a1; Bcl2a1a; Bfl-; Bfl-1; Bfl1; Hbp; Hbpa1; Hemopoietic-specific early response protein; Protein BFL-1
