Description
Product Usage Information
Western Blotting: 1:1000
Immunofluorescence (Immunocytochemistry): 1:800
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting, Immunofluorescence (Immunocytochemistry)
Specificity / Sensitivity
Phospho-Estrogen Receptor alpha (Ser167) (D5W3Z) Rabbit Monoclonal Antibody recognizes endogenous levels of ERα protein only when phosphorylated at Ser167.
Species Reactivity: Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser167 of human ERα protein.
Background
Estrogen receptor α (ERα), a member of the steroid receptor superfamily, contains highly conserved DNA binding and ligand binding domains (1). Through its estrogen-independent and estrogen-dependent activation domains (AF-1 and AF-2, respectively), ERα regulates transcription by recruiting coactivator proteins and interacting with general transcriptional machinery (2). Phosphorylation at multiple sites provides an important mechanism to regulate ERα activity (3-5). Ser104, 106, 118, and 167 are located in the amino-terminal transcription activation function domain AF-1, and phosphorylation of these serine residues plays an important role in regulating ERα activity. Ser118 may be the substrate of the transcription regulatory kinase CDK7 (5). Ser167 may be phosphorylated by p90RSK and Akt (4,6). According to the research literature, phosphorylation at Ser167 may confer tamoxifen resistance in breast cancer patients (4). ERα can be phosphorylated at Ser167 by various kinases such as S6K1, RSK, and Aurora A (7-9). Phosphorylation on Ser167 promotes ERα-dependent transcription and cellular proliferation, and is attributed to increased resistance to tamoxifen treatment (6, 9, 10). Various studies have shown that increased Ser167 phosphorylation correlates with poor prognosis in different cancer types (11, 12)
Alternate Names
DKFZp686N23123; E2 receptor alpha; ER; ER-alpha; Era; ESR; ESR1; ESRA; Estradiol receptor; estrogen nuclear receptor alpha; Estrogen receptor; estrogen receptor 1; estrogen receptor alpha delta 3; estrogen receptor alpha delta 4; estrogen receptor alpha delta 4 +49 isoform; estrogen receptor alpha E1-E2-1-2; estrogen receptor alpha E1-N2-E2-1-2; ESTRR; NR3A1; Nuclear receptor subfamily 3 group A member 1; oestrogen receptor alpha
