Description
Product Usage Information
Western Blotting: 1:1000
Immunoprecipitation: 1:50
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting, Immunoprecipitation
Specificity / Sensitivity
Phospho-FoxO3a (Ser425) (D5E9T) Rabbit Monoclonal Antibody recognizes endogenous levels of FoxO3a protein only when phosphorylated at Ser425. This antibody does not cross-react with FoxO1 nor FoxO4 proteins. This antibody may detect a non-specific band at 65 kDa and at 240 kDa.
Species Reactivity: Human, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser425 of human FoxO3a protein.
Background
The Forkhead family of transcription factors is involved in tumorigenesis of rhabdomyosarcoma and acute leukemias (1-3). Within the family, three members (FoxO1, FoxO4, and FoxO3a) have sequence similarity to the nematode orthologue DAF-16, which mediates signaling via a pathway involving IGFR1, PI3K, and Akt (4-6). Active forkhead members act as tumor suppressors by promoting cell cycle arrest and apoptosis. Increased expression of any FoxO member results in the activation of the cell cycle inhibitor p27 Kip1. Forkhead transcription factors also play a part in TGF-β-mediated upregulation of p21 Cip1, a process negatively regulated through PI3K (7). Increased proliferation results when forkhead transcription factors are inactivated through phosphorylation by Akt at Thr24, Ser256, and Ser319, which results in nuclear export and inhibition of transcription factor activity (8). Forkhead transcription factors can also be inhibited by the deacetylase sirtuin (SirT1) (9). ERK phosphorylates FoxO3a at Ser425, degrading FoxO3a via the ubiquitin-proteasome pathway. Research has shown that this phosphorylation event inhibits FoxO3a's function to induce cell death and thus promotes cell proliferation and tumorigenesis (10, 11).
Alternate Names
AF6q21; AF6q21 protein; DKFZp781A0677; FKHRL1; FKHRL1P2; forkhead box O3; forkhead box O3A; Forkhead box protein O3; forkhead homolog (rhabdomyosarcoma) like 1; Forkhead in rhabdomyosarcoma-like 1; forkhead, Drosophila, homolog of, in rhabdomyosarcoma-like 1; FOXO2; FOXO3; FOXO3A; MGC12739; MGC31925
