Description
Product Usage Information
For optimal ChIP results, use 2.5 μL of antibody and 10 μg of chromatin (approximately 4 × 10 6 cells) per IP. This antibody has been validated using SimpleChIP ® Enzymatic Chromatin IP Kits. The CUT&RUN dilution was determined using CUT&RUN Assay Kit # 86652 .
Western Blotting: 1:1000
Immunoprecipitation: 1:100
Chromatin IP: 1:200
CUT&RUN: 1:50
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting, Immunoprecipitation, Chromatin IP, CUT&RUN
Specificity / Sensitivity
LDB1 (E2Y9Q) Rabbit Monoclonal Antibody recognizes endogenous levels of total LDB1 protein.
Species Reactivity: Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human LDB1 protein.
Background
LIM domain-containing protein 1 (LDB1) is a nuclear adaptor protein and transcription cofactor that interacts with a multitude of LIM domain-containing transcription factors. Through such interactions, LDB1 facilitates promoter-enhancer bridging and is necessary for the activation and/or repression of genes in multiple cell lineages, including neuronal, cardiac, and hematopoietic cell lineages. Specifically, LDB1 associates with the T cell acute lymphocytic leukemia protein 1 (TAL1) and the nuclear adaptor LIM domain only protein 2 (LMO2) protein to regulate erythroid gene expression (1). LDB1 is crucial for hematopoietic development, and deletion of LDB1 results in embryonic lethality (2). LDB1 is also required for the maintenance of basal mammary epithelial stem cells and promotes breast tumorigenesis (3). In addition, LDB1 stabilizes LMO2 and is necessary to promote oncogenic properties of LMO2-driven leukemia (4). Structurally, LDB1 contains an amino-terminal homodimerization domain and a carboxy-terminal LIM interaction domain (LID). This protein has no known enzymatic or nucleic-acid binding functions (5).
Alternate Names
carboxy terminal LIM domain protein 2; Carboxyl-terminal LIM domain-binding protein 2; CLIM-2; CLIM2; hLdb1; LDB-1; LDB1; LIM domain binding 1; LIM domain-binding factor CLIM2; LIM domain-binding factor-1; LIM domain-binding protein 1; NLI; Nuclear LIM interactor
