Description
Product Usage Information
Immunohistochemistry (Paraffin): 1:50 - 1:200
Storage
Supplied in PBS (pH 7.2), less than 0.1% sodium azide, and 2 mg/mL BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.
Protocol
Available protocols: Immunohistochemistry (Paraffin)
Specificity / Sensitivity
MHC Class I (E8E7N) Rabbit Monoclonal Antibody (Alexa Fluor
Species Reactivity: Human, Mouse
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu254 of mouse H2-D1 protein.
Background
The human leukocyte antigen (HLA) system is a gene complex encoding the major histocompatibility complex (MHC) proteins in humans. These cell surface proteins are responsible for the regulation of antigen-specific immunity in humans. HLA genes are highly polymorphic, allowing them to fine-tune the adaptive immune response. HLAs corresponding to MHC class I (HLA-A, B, and C) present small peptide antigens from inside the cell, approximately 8 to 10 amino acids in length, to CD8 T lymphocytes in order to activate a cytotoxic T cell response. HLAs corresponding to MHC class II (HLA-DP, DM, DO, DQ, and DR) present antigens from outside of the cell, approximately 15 to 24 residues in length, to CD4 T helper cells, which in turn secrete cytokines and stimulate B cells to produce antibodies to that specific antigen. HLAs corresponding to MHC class III encode components of the complement system (1,2).
Alternate Names
H-2 class I histocompatibility antigen, D-B alpha chain; H-2D; H-2D cell surface glycoprotein; H-2D(B); H2-D; H2-D1; H2-DC1-beta transplantation antigen protein; H2-K1; HA11; histocompatibility 2, D region locus 1; MHC class I H-2Dp; MHC class I H2 antigen; MHC H2-D-q alpha-chain; Q5k protein
