Description
Product Usage Information
Immunohistochemistry (Paraffin): 1:100 - 1:400
Storage
Supplied in a Tris-based buffer with 1% BSA and less than 0.1% sodium azide. Stable for 24 months when stored at 4°C. Do not aliquot the antibody.
Protocol
Available protocols: Immunohistochemistry (Paraffin)
Specificity / Sensitivity
Perforin (IHC646) Mouse Monoclonal Antibody recognizes endogenous levels of total perforin protein.
Species Reactivity: Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a recombinant protein fragment corresponding to human perforin protein.
Background
Granzymes are a family of serine proteases expressed by cytotoxic T lymphocytes and natural killer (NK) cells and are key components of immune responses to pathogens and transformed cells (1). Granzymes are synthesized as zymogens and are processed into mature enzymes by cleavage of a leader sequence. They are released by exocytosis in lysosome-like granules containing perforin, a membrane pore-forming protein. Granzyme B has the strongest apoptotic activity of all the granzymes as a result of its caspase-like ability to cleave substrates at aspartic acid residues thereby activating procaspases directly and cleaving downstream caspase substrates (2,3). Perforin is a pore-forming protein that facilitates the entry of cytotoxic serine proteases, such as granzymes, into target cells (4). Perforin is primarily expressed in cytotoxic T lymphocytes and NK cells.
Alternate Names
Cytolysin; FLH2; HPLH2; lymphocyte pore forming protein; Lymphocyte pore-forming protein; MGC65093; P1; PERF; perforin; perforin 1; perforin 1 (pore forming protein); Perforin-1; PFN1; PFP; PRF1
