Description
Product Usage Information
Western Blotting: 1:1000
Immunoprecipitation: 1:100
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting, Immunoprecipitation
Specificity / Sensitivity
ZCCHC3 Antibody recognizes endogenous levels of total ZCCHC3 protein.
Species Reactivity: Human
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gln17 of human ZCCHC3 protein. Antibodies are purified by peptide affinity chromatography.
Background
ZCCHC3 belongs to the family of CCHC-type zinc-finger proteins (1). It binds to cGAS and acts as a cGAS co-sensor in recognizing double-stranded DNA (dsDNA), therefore, playing an essential role in innate immune response to DNA virus (2). ZCCHC3 was also shown to bind dsRNA and promote the binding of dsRNA by the retinoic acid-inducible gene-I (RIG-I)-like receptors (RLRs) RIG-I and MDA5, playing a critical role in RLR-mediated innate immune response to RNA virus (3).
Alternate Names
C20orf99; MGC104290; ZCCHC3; ZCHC3; Zinc finger CCHC domain-containing protein 3; zinc finger CCHC-type containing 3; zinc finger, CCHC domain containing 3
