Description
Product Usage Information
Western Blotting: 1:1000
IHC Leica Bond: 1:250 - 1:1000
Immunohistochemistry (Paraffin): 1:250 - 1:1000
Immunofluorescence (Immunocytochemistry): 1:50 - 1:200
Flow Cytometry (Fixed/Permeabilized): 1:100 - 1:400
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier-free (BSA and azide free) version of this product see product # 54370 .
Protocol
Available protocols: Western Blotting, IHC Leica Bond, Immunohistochemistry (Paraffin), Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed/Permeabilized)
Specificity / Sensitivity
CD10/Neprilysin (E5P7S) Rabbit Monoclonal Antibody recognizes endogenous levels of total CD10/Neprilysin protein.
Species Reactivity: Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with human CD10/Neprilysin recombinant protein.
Background
CD10, also known as Neprilysin, common acute lymphoblastic leukemia antigen (CALLA), or neutral endopeptidase (NEP), is a zinc-dependent transmembrane metallo-endopeptidase. CD10 cleaves peptides at the N-terminal side of hydrophobic amino acid residues and deactivates a variety of signaling peptides (1,2). CD10 was shown to be one of the markers for a natural killer cell-restricted progenitor in fetal and adult tissues (3). Studies also suggest CD10 as a phenotypic marker to distinguish mature neutrophils from immature neutrophils in patients with inflammation (2). In addition, CD10 GPR77 carcinoma-associated fibroblasts (CAFs) promote tumor formation and chemoresistance (4).
Alternate Names
Atriopeptidase; CALLA; CD10; CMT2T; Common acute lymphocytic leukemia antigen; DKFZp686O16152; Enkephalinase; EPN; membrane metallo-endopeptidase; membrane metallo-endopeptidase (neutral endopeptidase, enkephalinase, CALLA, CD10); membrane metallo-endopeptidase variant 1; membrane metallo-endopeptidase variant 2; membrane metalloendopeptidase; MGC126681; MGC126707; MME; NEP; Neprilysin; neprilysin-390; neprilysin-411; Neutral endopeptidase; Neutral endopeptidase 24.11; SCA43; SFE; Skin fibroblast elastase
