Description
Product Usage Information
Western Blotting: 1:1000
Immunoprecipitation: 1:50
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting, Immunoprecipitation
Specificity / Sensitivity
ATF-6 (D4Z8V) Rabbit Monoclonal Antibody recognizes endogenous levels of total ATF-6 alpha protein. This antibody does not detect ATF-6 beta protein.
Species Reactivity: Human, Mouse
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human ATF-6 alpha protein.
Background
Activating Transcription Factor 6 (ATF-6) is a transmembrane glycoprotein of the endoplasmic reticulum (ER). Upon accumulation of improperly folded proteins in the ER, ATF-6 is cleaved liberating a 50 kDa amino-terminal fragment that translocates to the nucleus (1, 2). There, ATF-6 activates transcription of ER chaperones and components of ER-associated degradation such as GRP78, XBP-1, and CHOP (3-5).
Alternate Names
ACHM7; activating transcription factor 6; Activating transcription factor 6 alpha; ATF6; ATF6-alpha; ATF6A; cAMP-dependent transcription factor ATF-6 alpha; Cyclic AMP-dependent transcription factor ATF-6 alpha; Processed cyclic AMP-dependent transcription factor ATF-6 alpha
