Description
Product Usage Information
Western Blotting: 1:1000
Immunofluorescence (Immunocytochemistry): 1:100 - 1:400
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting, Immunofluorescence (Immunocytochemistry)
Specificity / Sensitivity
PSPC1 (E7L8B) Rabbit Monoclonal Antibody recognizes endogenous levels of total PSPC1 protein. Faint bands running around 30 kDa, 80 kDa, and 100 kDa were observed. We are unclear of the identity of these bands.
Species Reactivity: Human, Mouse
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human PSPC1 protein.
Background
PSPC1 (paraspeckle component 1) was one of the first proteins found to be enriched in subnuclear bodies that are adjacent to but distinct from nuclear speckles, and subsequently named paraspeckles (1). PSPC1 belongs to the behavior protein family comprised of human splicing (DBHS) proteins, including SFPQ (splicing factor proline-glutamine rich ) and NONO (non-POU domain-containing octamer-binding protein) which are also enriched in paraspeckles (2). DBHS proteins are a unique family of RNA-binding proteins that recruit various proteins and nucleic acids involved in transcriptional activation and repression, transcript localization, splicing, and DNA damage repair (3). Important to disease, DBHS proteins bind the long noncoding RNA NEAT1 which is found to be enriched in paraspeckles. Aberrant accumulation of NEAT1 transcripts in paraspeckles has been identified in multiple tumor types, leading to a proposal that the long coding RNA, via the process of sponging, disrupts the ratio of miRNAs to target proteins, resulting in altered protein expression profiles that promote cancer (4).
Alternate Names
DKFZp566B1447; FLJ10955; Paraspeckle component 1; Paraspeckle protein 1; PSP1; PSPC1
