Description
Product Usage Information
Western Blotting: 1:1000
Simple Western™: 1:10 - 1:50
Immunofluorescence (Immunocytochemistry): 1:400 - 1:1600
Flow Cytometry (Fixed/Permeabilized): 1:400 - 1:1600
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting, Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed/Permeabilized)
Specificity / Sensitivity
OAT (F5L4R) Rabbit Monoclonal Antibody recognizes endogenous levels of total OAT protein.
Species Reactivity: Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala52 of human OAT protein.
Background
Mitochondrial ornithine aminotransferase (OAT) catalyzes the reversible conversion of L-ornithine and 2-oxoglutarate to L-glutamate 5-semialdehyde and L-glutamate (1). Studies show that pancreatic ductal adenocarcinoma (PDA) relies on ornithine synthesis from glutamine, a process mediated by OAT. Ornithine derived from glutamine is used for polyamine synthesis and is critical for PDA growth. In contrast, most adult normal tissues are dependent on ornithine derived from arginine for polyamine synthesis (2). In addition, it was shown that soluble dietary fiber remodels gut microbiota composition and enhances the abundance of , which promotes OAT expression in hepatocytes. OAT facilitates ammonia detoxification in the liver and, therefore, reduces alcohol-induced liver injury in mice (3).
Alternate Names
DKFZp781A11155; GACR; gyrate atrophy; HOGA; OAT; OATASE; OKT; ornithine aminotransferase; Ornithine aminotransferase, hepatic form; Ornithine aminotransferase, mitochondrial; Ornithine aminotransferase, renal form; Ornithine delta-aminotransferase; Ornithine--oxo-acid aminotransferase; ornithine-delta-aminotransferase; ornithine-oxo-acid aminotransferase; testicular tissue protein Li 130
