Description
Product Usage Information
Western Blotting: 1:1000
Storage
Supplied in PBS with less than 0.1% sodium azide. Stable for 12 months when stored at -20ºC. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present, but will not interfere with antibody performance.
Protocol
Available protocols: Western Blotting
Specificity / Sensitivity
Ubiquitin (Ubi-1) Mouse Monoclonal Antibody recognizes endogenous levels of total ubiquitin protein.
Species Reactivity: All Species Expected
Source / Purification
Monoclonal antibody is produced by immunizing animals with purified bovine ubiquitin conjugated to carrier protein.
Background
Ubiquitin is a conserved polypeptide unit that plays an important role in the ubiquitin-proteasome pathway. Ubiquitin can be covalently linked to many cellular proteins by the ubiquitination process, which targets proteins for degradation by the 26S proteasome. Three components are involved in the target protein-ubiquitin conjugation process. Ubiquitin is first activated by forming a thiolester complex with the activation component E1; the activated ubiquitin is subsequently transferred to the ubiquitin-carrier protein E2, then from E2 to ubiquitin ligase E3 for final delivery to the epsilon-NH of the target protein lysine residue (1-3). The ubiquitin-proteasome pathway has been implicated in a wide range of normal biological processes and in disease-related abnormalities. Several proteins such as IκB, p53, cdc25A, and Bcl-2 have been shown to be targets for the ubiquitin-proteasome process as part of regulation of cell cycle progression, differentiation, cell stress response, and apoptosis (4-7).
Alternate Names
40S ribosomal protein S27a; 60S ribosomal protein L40; CEP52; CEP80; epididymis luminal protein 112; epididymis secretory protein Li 50; FLJ25987; HEL-S-50; HEL112; HMG20; HUBCEP52; L40; Large ribosomal subunit protein eL40; MGC126879; MGC126881; MGC57125; MGC8385; polyubiquitin B; Polyubiquitin-B; Polyubiquitin-C; ribosomal protein L40; ribosomal protein S27a; RL40; RPL40; RPS27A; RS27A; S27A; Small ribosomal subunit protein eS31; UBA52; UBA80; UBB; UBC; UBCEP1; UBCEP2; UBCEP80; ubiquitin; Ubiquitin; ubiquitin A-52 residue ribosomal protein fusion product 1; ubiquitin and ribosomal protein L40; ubiquitin and ribosomal protein S27a; Ubiquitin B; ubiquitin C; ubiquitin carboxyl extension protein 52; Ubiquitin carboxyl extension protein 80; Ubiquitin-40S ribosomal protein S27a; ubiquitin-52 amino acid fusion protein; Ubiquitin-60S ribosomal protein L40; ubiquitin-CEP52; ubiquitin-CEP80; Ubiquitin-ribosomal protein eL40 fusion protein; Ubiquitin-ribosomal protein eS31 fusion protein
