Description
Product Usage Information
Western Blotting: 1:1000
Immunoprecipitation: 1:50
Immunohistochemistry (Paraffin): 1:400 - 1:1600
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 78328 .
Protocol
Available protocols: Western Blotting, Immunoprecipitation, Immunohistochemistry (Paraffin)
Specificity / Sensitivity
Claudin-5 (E5D9Y) Rabbit Monoclonal Antibody recognizes endogenous levels of total claudin-5 protein. Species reactivity for immunohistochemistry is human only. Non-specific staining was observed in skeletal muscle by immunohistochemistry.
Species Reactivity: Human, Mouse
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala202 of human claudin-5 protein.
Background
Tight junctions, or zonula occludens, form a continuous barrier to fluids across the epithelium and endothelium. They function in regulation of paracellular permeability and in the maintenance of cell polarity, blocking the movement of transmembrane proteins between the apical and the basolateral cell surfaces. Tight junctions are composed of claudin and occludin proteins, which join the junctions to the cytoskeleton (1,2). The claudin family is composed of 23 integral membrane proteins, and their expression, which varies among tissue types, may determine both the strength and properties of the epithelial barrier. Alteration in claudin protein expression pattern is associated with several types of cancer (2,3). Claudin-5, highly expressed in brain and lung tissue, is a primary component of the tight junctions of blood-brain barrier endothelial cells. The blood-brain barrier regulates homeostatic and pathological conditions in the brain by limiting passage of material circulating to the brain. The blood-brain barrier can also impede the effectiveness of small molecule therapeutics (4). Claudin-5 is also thought to regulate tumor cell motility brain metastasis (5).
Alternate Names
androgen withdrawal and apoptosis induced protein RVP1-like; AWAL; BEC1; claudin 5; Claudin-5; claudin5; CLD5; CLDN5; CPETRL1; TMDVCF; TMVCF; Transmembrane protein deleted in VCFS; transmembrane protein deleted in velocardiofacial syndrome
