Description
Product Usage Information
Western Blotting: 1:1000
Storage
Supplied in 140 mM NaCl, 3 mM KCI, 10 mM sodium phosphate (pH 7.4) dibasic, 2 mM potassium phosphate monobasic, 2 mg/mL BSA, and 50% glycerol. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting
Specificity / Sensitivity
TXNIP (D5F3E) Rabbit Monoclonal Antibody (Biotinylated) recognizes endogenous levels of total TXNIP protein.
Species Reactivity: Human, Mouse, Rat, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val337 of human TXNIP protein.
Background
The ubiquitously expressed thioredoxin-interacting protein (TXNIP) binds and inhibits thioredoxin to regulate cellular redox state (1-3). Research studies demonstrate that hyperglycemia induces TXNIP expression and increases cellular oxidative stress (1). In addition, these studies show that TXNIP reduces glucose uptake directly by binding the glucose transporter Glut1 to stimulate receptor internalization or indirectly by reducing Glut1 mRNA levels (3). Additional studies indicate that TXNIP plays a role in the regulation of insulin mRNA transcription (4). Microarray analyses indicate that TXNIP acts downstream of PPARγ and is a putative tumor suppressor that may control thyroid cancer cell progression (5). In addition, the TXNIP protein may be a potential therapeutic target for the treatment of type 2 diabetes and some disorders related to ER-stress (6).
Alternate Names
ARRDC6; EST01027; HHCPA78; THIF; thioredoxin binding protein 2; thioredoxin interacting protein; Thioredoxin-binding protein 2; Thioredoxin-interacting protein; TXNIP; upregulated by 1,25-dihydroxyvitamin D-3; VDUP1; Vitamin D3 up-regulated protein 1
