Description
Product Usage Information
Immunoprecipitation: 1:20
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Immunoprecipitation
Specificity / Sensitivity
V5-Tag (D3H8Q) Rabbit mAb detects recombinant proteins containing the V5 epitope tag. The antibody recognizes the V5-tag fused to either the amino or carboxy terminus of targeted proteins in transfected cells. V5-Tag (D3H8Q) Rabbit mAb detects exogenously expressed V5-tagged proteins in cells expressed under a CMV promoter. Expression under other promoters has not been evaluated.
Species Reactivity: All Species Expected
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic V5-tag peptide.
Background
Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein's biochemical properties. The V5-tag is a 14 amino acid peptide derived from a small epitope (Pk) on the P and V proteins of simian virus 5 (SV5), a member of the paramyxovirus family (1). The V5-tag can be used to detect expression of recombinant proteins in bacteria, yeast, insects, and mammalian cell systems.
