Description
Product Usage Information
Western Blotting: 1:1000
Simple Western™: 1:50 - 1:250
Immunoprecipitation: 1:50
Immunohistochemistry (Paraffin): 1:200 - 1:800
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 82819 .
Protocol
Available protocols: Western Blotting, Immunoprecipitation, Immunohistochemistry (Paraffin)
Specificity / Sensitivity
Ras (E8N8L) Rabbit Monoclonal Antibody detects endogenous levels of total K-Ras, H-Ras, and N-Ras proteins.
Species Reactivity: Human, Mouse, Rat, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Glu37 of human K-Ras protein.
Background
The 21 kDa guanine-nucleotide binding proteins (K-Ras, H-Ras, and N-Ras) cycle between active (GTP-bound) and inactive (GDP-bound) forms (1). Receptor tyrosine kinases and G protein-coupled receptors activate Ras, which then stimulates the Raf-MEK-MAPK pathway (2-4). GTPase-activating proteins (GAPs) normally facilitate the inactivation of Ras. However, research studies have shown that in 30% of human tumors, point mutations in Ras prevent the GAP-mediated inhibition of this pathway (5). The most common oncogenic Ras mutation found in tumors is Gly12 to Asp12 (G12D), which prevents Ras inactivation, possibly by increasing the overall rigidity of the protein (5,6).
Alternate Names
'C-K-RAS; ALPS4; C-BAS/HAS; c-H-ras; C-HA-RAS1; c-has/bas p21 protein; c-K-ras; c-Ki-ras; c-Ki-ras2; c-Kirsten-ras protein; c-ras-Ki-2 activated oncogene; cellular c-Ki-ras2 proto-oncogene; cellular transforming proto-oncogene; CFC2; CMNS; CTLO; GTP- and GDP-binding peptide B; GTPase HRas; GTPase HRas, N-terminally processed; GTPase KRas; GTPase KRas, N-terminally processed; GTPase NRas; H-Ras; H-Ras-1; H-RASIDX; Ha-Ras; Ha-Ras1 proto-oncoprotein; HAMSV; Harvey rat sarcoma viral oncogene homolog; Harvey rat sarcoma viral oncoprotein; HRAS; HRas proto-oncogene, GTPase; HRAS1; K-Ras; K-RAS; K-Ras 2; K-ras p21 protein; K-RAS2A; K-RAS2B; K-RAS4A; K-RAS4B; Ki-Ras; Kirsten rat sarcoma viral oncogene homolog; Kirsten rat sarcoma viral proto-oncogene; Kirsten rat sarcoma-2 viral (v-Ki-ras2) oncogene homolog; KRAS; KRAS proto-oncogene, GTPase; KRAS1; KRAS2; N-ras; N-RAS; N-ras protein part 4; NCMS; neuroblastoma RAS viral (v-ras) oncogene homolog; neuroblastoma RAS viral oncogene homolog; NRAS; NRAS proto-oncogene, GTPase; NRAS1; NS; NS3; NS6; OES; oncogene KRAS2; p19 H-RasIDX protein; p21ras; PR310 c-K-ras oncogene; RALD; Ras family small GTP binding protein H-Ras; RASH; RASH1; RASK; RASK2; RASN; transformation gene: oncogene HAMSV; Transforming protein N-Ras; transforming protein p21; Transforming protein p21; v-Ha-ras Harvey rat sarcoma viral oncogene homolog; v-Ki-ras2 Kirsten rat sarcoma 2 viral oncogene homolog; v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog; v-ras neuroblastoma RAS viral oncogene homolog
