Description
Product Usage Information
Western Blotting: 1:1000
Immunoprecipitation: 1:50
Immunofluorescence (Immunocytochemistry): 1:50
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 37080 .
Protocol
Available protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Immunocytochemistry)
Specificity / Sensitivity
Syntaxin-4 (E6W7B) Rabbit Monoclonal Antibody recognizes endogenous levels of total Syntaxin-4 protein. This antibody may also detect a putative SDS-resistant Syntaxin-4 protein-containing (SNARE) complex at approximately 65 kDa.
Species Reactivity: Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human Syntaxin-4 protein.
Background
Proteins in the soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) complex are integral membrane proteins involved in intracellular vesicle trafficking and exocytosis, and are essential for processes that require rapid, targeted, and regulated membrane fusion such as cell growth, hormone secretion, and neurotransmission (reviewed in 1,2). Functioning as a target or t-SNARE protein, Syntaxin-4 is characterized by a long cytoplasmic N-terminal region encompassing two coiled-coil domains, a single transmembrane domain, and a very short extracellular C-terminus (3). Syntaxin-4 is known to regulate glucose uptake in skeletal muscle and adipose tissue by facilitating the translocation of the glucose transporter GLUT4 from an intracellular compartment to the plasma membrane (4,5). Moreover, human islet β-cells overexpressing exhibited enhanced insulin secretory capability, resilience against proinflammatory cytokine-induced apoptosis, and reduced chemokine expression (6). Additionally, research studies have indicated that Syntaxin-4 plays a role in the exocytosis of AMPA receptors in hippocampal neurons (7), lytic granule exocytosis in cytotoxic T-lymphocytes (8), and hepatitis C viral particle release (9).
Alternate Names
p35-2; Renal carcinoma antigen NY-REN-31; STX4; STX4A; syntaxin 4; syntaxin 4A (placental); Syntaxin-4
