Product Usage Information
For optimal flow cytometry results, we recommend 0.06 μg of antibody per test.
Flow Cytometry (Fixed/Permeabilized): 1:300
Flow Cytometry (Live): 1:300
Storage
Supplied in 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH 7.2. This product is stable for 12 months when stored at 4ºC. Do not aliquot the antibody. Protect from light. Do not freeze.
Protocol
Available protocols: Flow Cytometry (Fixed/Permeabilized), Flow Cytometry (Live)
Specificity / Sensitivity
MHC Class II (I-A/I-E) (M5/114.15.2) Rat Monoclonal Antibody (PE Conjugate) recognizes endogenous levels of total MHC class II (I-A/I-E) proteins. This antibody conjugate detects epitopes within the extracellular domain of MHC class II (I-A/I-E).
Species Reactivity: Mouse
Source / Purification
This monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.
Background
Major histocompatibility complex class II (MHC class II) molecules are heterodimeric, transmembrane glycoproteins expressed on the surface of antigen-presenting cells, such as macrophages, dendritic cells, and B cells. Expression can also be induced on other cell types through interferon-γ signaling (1). Prior to being displayed on the cell membrane, MHC class II molecules are loaded with exogenous peptide antigens approximately 15-24 amino acids in length that were derived from endocytosed extracellular proteins digested in the lysosome (2). Antigen-presentation through MHC class II is required for T cell activation during the immune response to extracellular pathogens (2). In humans, the MHC class II protein complex is encoded by the human leukocyte antigen gene complex (HLA). HLAs corresponding to MHC class II are HLA-DP, HLA-DM, HLA-DOA, HLA-DOB, HLA-DQ, and HLA-DR (3). The M5/114.15.2 antibody reacts with mouse MHC class II, both I-A and I-E subregion-encoded glycoproteins (I-Ab, I-Ad, I-Aq, I-Ed, I-Ek, not I-Af, I-Ak, or I-As). It detects a polymorphic determinant present on B cells, monocytes, macrophages, dendritic cells, and activated T lymphocytes from mice carrying the H-2b, H-2d, H-2q, H-2p, H-2r and H-2u haplotypes, but not from mice carrying the H-2s or H-2f haplotypes (4-7). The M5/114 mAb is reported to inhibit I-A-restricted T cell responses of the H-2b, H-2d, H-2q, H-2u but not H-2f, H-2k, or H-2s haplotypes (8,9).
Alternate Names
A alpha; A beta; A130038H09Rik; Aalpha; Abe; Abeta; AI845868; cell surface glycoprotein; E-beta-b; E-W17; H-2; H-2 class II histocompatibility antigen E-a/k beta-2; H-2 class II histocompatibility antigen, A beta chain; H-2 class II histocompatibility antigen, A-B alpha chain; H-2 class II histocompatibility antigen, A-D alpha chain; H-2 class II histocompatibility antigen, A-D beta chain; H-2 class II histocompatibility antigen, A-F alpha chain; H-2 class II histocompatibility antigen, A-F beta chain; H-2 class II histocompatibility antigen, A-K alpha chain; H-2 class II histocompatibility antigen, A-K beta chain; H-2 class II histocompatibility antigen, A-Q alpha chain; H-2 class II histocompatibility antigen, A-Q beta chain; H-2 class II histocompatibility antigen, A-R alpha chain; H-2 class II histocompatibility antigen, A-S alpha chain; H-2 class II histocompatibility antigen, A-S beta chain; H-2 class II histocompatibility antigen, A-U alpha chain; H-2 class II histocompatibility antigen, A-U beta chain; H-2 class II histocompatibility antigen, E-B beta chain; H-2 class II histocompatibility antigen, E-D beta chain; H-2 class II histocompatibility antigen, E-Q beta chain; H-2 class II histocompatibility antigen, E-S beta chain; H-2 class II histocompatibility antigen, I-A beta chain; H-2 class II histocompatibility antigen, I-E alpha chain; H-2 class II histocompatibility antigen, I-E beta chain; H-2 I-A beta p; H-2A; H-2Aa; H-2Ab; H-2E beta; H-2Eb; H-2Eb2; H2-A; H2-Aa; H2-Ab; H2-Ab_; H2-Ab1; H2-Eb_p; H2-Eb1; H2-Eb2; H2Aa; H2Eb; HA2F; HB2I; HB2Q; histocompatibility 2, class II antigen A, alpha; histocompatibility 2, class II antigen A, alpha, promoter 1; histocompatibility 2, class II antigen A, beta 1; histocompatibility 2, class II antigen A, beta, promoter 1; histocompatibility 2, class II antigen E beta; histocompatibility 2, class II antigen E beta, promoter 1; Histocompatibility 2, class II antigen E beta2; HLA-DQA1; HLA-DQB1; HLA-DRB1; I-A beta MHC class II; I-A<; I-A; I-Ab; I-Aalpha; I-Ab; I-Abeta; I-E beta MHC class II; I-E beta-chain; Ia-1; Ia-2; Ia-4; Ia-5; Ia1; Ia2; Ia4; Ia5; IAalpha; IAb; Iebeta2; lymphocyte antigen; major histocompatibility complex class II beta chain; major histocompatibility complex H-2E beta chain; major histocompatibility complex H-2E beta chain'; major histocompatibility protein class II alpha chain; MGC163794; MGC163796; MHC class II antigen; MHC class II antigen A beta; MHC class II antigen alpha chain; MHC class II antigen beta chain; MHC class II antigen E beta2; MHC class II beta chain A-beta-5; MHC class II beta chain A-beta-7; MHC class II E beta; MHC class II E-beta-2; MHC class II H2-IA-beta-psi; MHC class II IE-b; MHC class II protein; MHC E-beta-f; MHC H2-IA-beta-1 (bm12) chain; MHC H2-IE-beta cell surface glycoprotein; MHC II H2-IE-beta; MHC-II I-A(d) alpha; MHC-II I-A(d) beta; response to metastatic cancers 1; Rmc; Rmcs1
