Description
Product Usage Information
Western Blotting: 1:1000
Immunohistochemistry (Paraffin): 1:200 - 1:800
Flow Cytometry (Fixed/Permeabilized): 1:400 - 1:1600
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 33484 .
Protocol
Available protocols: Western Blotting, Immunohistochemistry (Paraffin), Flow Cytometry (Fixed/Permeabilized)
Specificity / Sensitivity
IL-18 (E3G8R) Rabbit Monoclonal Antibody recognizes endogenous levels of total IL-18 protein.
Species Reactivity: Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with human IL-18 recombinant protein.
Background
Interleukin-18 (IL-18), originally known as interferon-gamma inducing factor (IGIF), is part of the IL-superfamily of cytokines (1,2). This proinflammatory cytokine is synthesized as an inactive precursor which requires cleavage by Caspase-1 to become an active, mature molecule, which is secreted by monocytes and macrophages (3,4,6). It induces IFNγ production in T-helper-1 (Th1) cells and natural killer (NK) cells, and can regulate innate immunity via both Th1 and Th2 responses (4,5). Elevated IL-18 levels are associated with autoimmune disease, but may be balanced by binding to IL-18 binding protein (IL-18BP), which along with IL-18 neutralizing antibodies, are being examined in clinical trials (6,7).
Alternate Names
Iboctadekin; IFN-gamma-inducing factor; IGIF; IL 18; IL-1 gamma; IL-18; IL-1g; IL18; IL1F4; Interferon gamma-inducing factor; interleukin 18; interleukin 18 (interferon-gamma-inducing factor); Interleukin-1 gamma; Interleukin-18; MGC12320
