Description
Product Usage Information
For optimal ChIP results, use 10 μL of antibody and 10 μg of chromatin (approximately 4 x 10 6 cells) per IP. This antibody has been validated using SimpleChIP ® Enzymatic Chromatin IP Kits.
Western Blotting: 1:1000
Immunoprecipitation: 1:200
Chromatin IP: 1:200
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting, Immunoprecipitation, Chromatin IP
Specificity / Sensitivity
DGCR8 (E9O3L) Rabbit Monoclonal Antibody recognizes endogenous levels of total DGCR8 protein.
Species Reactivity: Human, Mouse, Rat, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro767 of mouse DGCR8 protein.
Background
Drosha was identified as a nuclear RNase III that catalyzes the initial step of microRNA (miRNA) processing (1). This enzyme processes the long primary transcript pri-miRNAs into stem-looped pre-miRNAs. Interference of Drosha results in the increase of pri-miRNAs and the decrease of pre-miRNAs (1). Drosha exists in a multiprotein complex called Microprocessor along with other components, such as DGCR8 (2). Drosha and DGCR8 are necessary for miRNA biogenesis (3).
Alternate Names
D16H22S1742E; D16H22S788; D16H22S788E; D16Wi; D16Wis2; Dgcr8; DGCR8, microprocessor complex subunit; DiGeorge syndrome critical region 8 homolog; DiGeorge syndrome critical region gene 8; Gy1; Microprocessor complex subunit DGCR8; mir-1306; N41; OTTMUSP00000027994; OTTMUSP00000027995; Vo59c; Vo59c07
