Description
Product Usage Information
For optimal ChIP results, use 10 μL of antibody and 10 μg of chromatin (approximately 4 × 10 6 cells) per IP. This antibody has been validated using SimpleChIP ® Enzymatic Chromatin IP Kits.
Western Blotting: 1:1000
Chromatin IP: 1:100
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting, Chromatin IP
Specificity / Sensitivity
BRD1 (F8X6N) Rabbit Monoclonal Antibody recognizes endogenous levels of total BRD1 protein.
Species Reactivity: Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant protein specific to human BRD1 protein.
Background
Bromodomain-containing protein 1 (BRD1), also known as BRPF2, is part of a histone acetyltransferase complex along with HBO1 that is responsible for global acetylation of Lys14 on histone H3 (1). BRD1 contains a unique PHD finger domain with a positively charged surface, which can bind DNA non-specifically via interaction with histone H3 (2,3). By driving histone acetylation around key genes, BRD1 has been implicated in many cellular functions, including stem cell differentiation, T-cell development, and erythropoiesis (1,4,5). The gene has been shown to confer susceptibility to schizophrenia and bipolar disorder (6,7). BRD1 overexpression has been described in colorectal cancer, and BRD1 inhibition has been shown to increase immune therapy efficacy in high-grade ovarian cancer (8,9).
Alternate Names
BR140-like protein; BRD1; BRL; Bromodomain and PHD finger-containing protein 2; bromodomain containing 1; Bromodomain-containing protein 1; BRPF1; BRPF2; DKFZp686F0325
