Description
Product Usage Information
Western Blotting: 1:1000
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting
Specificity / Sensitivity
Methionine Synthase (D3D6H) Rabbit Monoclonal Antibody recognizes endogenous levels of total methionine synthase protein.
Species Reactivity: Human, Mouse, Rat, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro1178 of human methionine synthase protein.
Background
One-carbon metabolism includes enzymatic reactions involving the transfer of one-carbon groups mediated by folate cofactor. The activated one-carbon groups are used by various metabolic pathways, including purine synthesis, thymidine synthesis, and remethylation of homocysteine to methionine (1). One of the enzymes in one-carbon metabolism, methionine synthase, catalyzes the conversion of 5-methyltetrahydrofolate and homocysteine to tetrahydrofolate and methionine. Methionine is further converted to S-adenosylmethionine (SAM) (1, 2). S-adenosylmethionine (SAM) is a major reactive methyl carrier and plays a critical role in epigenetic regulation (2, 3).
Alternate Names
5-methyltetrahydrofolate--homocysteine methyltransferase; 5-methyltetrahydrofolate-homocysteine methyltransferase; 5-methyltetrahydrofolate-homocysteine methyltransferase 1; cblG; cobalamin-dependent methionine synthase; FLJ33168; FLJ43216; FLJ45386; HMAG; METH; Methionine synthase; MS; MTR; Vitamin-B12 dependent methionine synthase
