Description
Product Usage Information
Western Blotting: 1:1000
Immunoprecipitation: 1:50
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, and 50% glycerol. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting, Immunoprecipitation
Specificity / Sensitivity
Cytochrome b Antibody recognizes endogenous levels of total cytochrome b protein.
Species Reactivity: Human, Monkey
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro208 of human cytochrome b protein. Antibodies are purified by peptide affinity chromatography.
Background
Cytochrome b, encoded by the gene , is a subunit of ubiquinol-cytochrome c reductase, also known as cytochrome bc1, or complex III, of the mitochondrial respiratory (electron transport) chain (1). This protein complex mediates electron transfer from ubiquinol to cytochrome c, contributing to the generation of a proton gradient that powers ATP synthesis (2). Cytochrome b is an integral membrane protein and notable for being the only protein within complex III that is encoded by the mitochondrial genome. Genetic sequence analyses have identified mutations in  that are linked to a variety of diseases and pathologies (3,4). Natural variations in the sequence of  have also proven highly useful as a tool to determine phylogenetic relationships between organisms (5).
Alternate Names
COB; Complex III subunit 3; Complex III subunit III; CYB; CYTB; Cytochrome b; cytochrome b (mitochondrion); Cytochrome b-c1 complex subunit 3; mitochondrially encoded cytochrome b; MT-CYB; MTCYB; Ubiquinol-cytochrome-c reductase complex cytochrome b subunit
