Description
Product Usage Information
Western Blotting: 1:1000
IHC Leica Bond: 1:400 - 1:1600
Immunohistochemistry (Paraffin): 1:400 - 1:1600
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting, IHC Leica Bond, Immunohistochemistry (Paraffin)
Specificity / Sensitivity
CD70 (E3Q1A) Rabbit Monoclonal Antibody recognizes endogenous levels of total CD70 protein.
Species Reactivity: Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the carboxy terminus of human CD70 protein.
Background
CD70 is a type II transmembrane glycoprotein and a member of the tumor necrosis factor ligand superfamily (TNFSF), also known as CD27L and TNFSF7. It is normally expressed on medullary thymic epithelial cells. Its expression is induced on activated lymphoid cells (B cells, T cells, and NK cells) and dendritic cells. CD70 is a ligand for CD27, a co-stimulatory receptor that plays an important role in T cell activation and proliferation (1,2). CD70 overexpression has been reported in various tumors such as renal cell carcinoma, glioblastoma, and non-small cell lung carcinoma and it's being actively pursued as a therapeutic target (3-6).
Alternate Names
CD27 ligand; CD27-L; CD27L; CD27LG; CD70; CD70 antigen; CD70 molecule; Ki-24 antigen; LPFS3; surface antigen CD70; TNFSF7; TNLG8A; tumor necrosis factor (ligand) superfamily, member 7; tumor necrosis factor ligand 8A; Tumor necrosis factor ligand superfamily member 7; tumor necrosis factor ligand superfamily, member 7
