Description
Product Usage Information
Western Blotting: 1:1000
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting
Specificity / Sensitivity
Phospho-TBC1D1 (Ser700) Antibody detects transfected levels of TBC1D1 protein when phosphorylated at Ser700.
Species Reactivity: Mouse
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser700 of mouse TBC1D1 protein. Antibodies are purified by protein A and peptide affinity chromatography.
Background
TBC1D1 is a paralog of AS160 (1) and both proteins share about 50% identity (2). TBC1D1 was shown to be a candidate gene for severe obesity (3). It plays a role in Glut4 translocation through its GAP activity (2,4). Studies indicate that TBC1D1 is highly expressed in skeletal muscle (1). Insulin, AICAR, and contraction directly regulate TBC1D1 phosphorylation in this tissue (1). Three AMPK phosphorylation sites (Ser231, Ser660, and Ser700) and one Akt phosphorylation site (Thr590) were identified in skeletal muscle (5). Muscle contraction or AICAR treatment increases phosphorylation on Ser231, Ser660, and Ser700 but not on Thr590; insulin increases phosphorylation on Thr590 only (5).
Alternate Names
1110062G02Rik; AI385682; AW555803; Kiaa1108; MGC106104; MGC144097; mKIAA1108; Nob; Nob1; Nobq1; Tbc1; TBC1 domain family member 1; TBC1 domain family, member 1; Tbc1d1; TBCD1; tre-2/USP6, BUB2, cdc16) domain family, member 1
