CST, 69310S, Whitlow/218 Linker (E3U7Q) Rabbit Monoclonal Antibody (Alexa Fluor ® 647 Conjugate)

Product Usage Information
Flow Cytometry (Live): 1:50
Storage
Supplied in PBS (pH 7.2), less than 0.1% sodium azide, and 2 mg/mL BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.
Protocol
Available protocols: Flow Cytometry (Live)
Specificity / Sensitivity
Whitlow/218 Linker (E3U7Q) Rabbit Monoclonal Antibody (Alexa Fluor
Species Reactivity: All Species Expected
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues of the Whitlow/218 linker peptide.
Background
The Whitlow/218 peptide is a commonly used linker that connects the variable heavy (VH) domain and variable light (VL) domain of single-chain variable fragments (scFvs) and chimeric antigen receptors (CARs) that utilize an extracellular domain scFv for target antigen recognition. The Whitlow/218 linker affords the scFv with enhanced resistance to proteolysis and aggregation (1).