Description
Product Usage Information
Immunohistochemistry (Paraffin): 1:50 - 1:200
Storage
Supplied in PBS (pH 7.2), less than 0.1% sodium azide, and 2 mg/mL BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.
Protocol
Available protocols: Immunohistochemistry (Paraffin)
Specificity / Sensitivity
MAdCAM-1 (E1V8F) Rabbit Monoclonal Antibody (Alexa Fluor
Species Reactivity: Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human MAdCAM-1 protein.
Background
Mucosal addressin cell adhesion molecule 1 (MAdCAM-1) is a glycoprotein expressed by endothelial cells at venules of Peyer's patches, intestinal lamina propria, and mesenteric lymph nodes (1). MAdCAM-1 contributes to lymphocyte homing to these tissues, serving as a ligand for α4β7, L-Selectin, and, to a lesser extent, α4β1 (2-6). Expression of MAdCAM-1 is upregulated in patients with inflammatory bowel disease, and blocking MAdCAM-1 reduces colon inflammation by disrupting lymphocyte homing to the intestinal tract (7). In ulcerative colitis, disease activity may be a result of the L-Selectin ligand carbohydrates attached to MAdCAM-1 by -acetylglucosamine-6- -sulfotransferase-1 (GlcNAc6ST-1) rather than the expression levels of MAdCAM-1 alone (4).
Alternate Names
hMAdCAM-1; MACAM1; MADCA; MAdCAM-1; MADCAM1; Mucosal addressin cell adhesion molecule 1; mucosal addressin cell adhesion molecule-1; mucosal vascular addressin cell adhesion molecule 1
