Description
Product Usage Information
Western Blotting: 1:1000
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting
Specificity / Sensitivity
COPS6 Antibody recognizes endogenous levels of total COPS6 protein. This antibody also cross-reacts with an unidentifed protein of 78 kDa protein in some cell extracts.
Species Reactivity: Human, Mouse, Rat
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human COPS6 protein. Antibodies are purified by protein A and peptide affinity chromatography.
Background
COP9 signalosome complex subunit 6 (COPS6) belongs to the multisubunit COP9 signalsome which modulates protein ubiquitination to affect cellular processes such as cell-cycle progression and DNA damage repair (1). COPS6 is a putative oncogene that has been shown to be overexpressed in a significant percentage of colorectal cancers with poor prognosis (2). Mechanistically, research studies have shown that a signaling axis involving EGFR and ERK promotes tumor growth through the phosphorylation of COPS6, which indirectly facilitates downstream stabilization of β-catenin and enhancement of its transcriptional acitivity (2). Reasearch studies have also demonstrated that COPS6 contributes to tumorigenesis through the stabilization of other oncoproteins like c-Myc, MDM2 and E6AP (3-5).
Alternate Names
COP9 constitutive photomorphogenic homolog subunit 6; COP9 constitutive photomorphogenic homolog subunit 6 (Arabidopsis); COP9 signalosome complex subunit 6; COP9 signalosome subunit 6; COP9 subunit 6 (MOV34 homolog, 34 kD); COPS6; CSN6; H_NH0506M12.12; HVIP; JAB1-containing signalosome subunit 6; MOV34 homolog; MOV34 homolog, 34 kD; MOV34-34KD; SGN6; Signalosome subunit 6; Vpr-interacting protein
