Description
Product Usage Information
Immunoprecipitation: 1:100
Immunofluorescence (Frozen): 1:100
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Immunoprecipitation, Immunofluorescence (Frozen)
Specificity / Sensitivity
YKL-40 (F9N3K) Rabbit mAb recognizes endogenous levels of total YKL-40 protein.
Species Reactivity: Mouse
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of mouse YKL-40 protein.
Background
YKL-40, also known as chitinase-3-like protein 1 (CHI3L1), is a secreted glycoprotein encoded by the gene (1,2). Unlike other members of the chitinase-like protein family, YKL-40 is not an active chitinase. It is produced by activated macrophages, chondrocytes, neutrophils, and synovial cells. It is a pro-inflammatory molecule that contributes to the progression of many inflammatory diseases, including fibrosis, neurodegenerative disease, breast, lung, prostate, liver, bladder, colon, and other types of cancers (3,4). Levels of YKL-40 in serum and plasma in patients with various types of tumors have been reported to predict poor prognosis, and in some clinical assays, YKL-40 is being used as a predictive biomarker of cancer outcome (4).
Alternate Names
AW208766; Breast regression protein 39; Brp; Brp39; BRP39 protein; Cartilage glycoprotein 39; CGP-39; CH3L1; Chi3; Chi3l1; Chil1; chitinase 3 like 1; chitinase 3-like 1; Chitinase-3-like protein 1; chitinase-like 1; GP-39; Gp3; Gp39
