CST, 7002P6, STOP Solution
Detection System for studying in the research area.
Catalog Number: 7002P6
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Category
Manufacturer's Pagewww.cellsignal.com/products/buffers-dyes/stop-solution/7002
Description
Product Usage Information
(a) After incubation with horseradish peroxidase (HRP) conjugated secondary antibody, wash plates with PBS/0.05% Tween-20. (b) Add 100 μl of TMB solution/well. Incubate for a specific amount of time or until desired signal is achieved. See product datasheet for recommended incubation times for specific antibody pair. (c) Add 100 μl of STOP Solution. (d) Read at Absorbance 450 nm.
Storage
Store at 4ºC.
Background
STOP Solution is used to terminate TMB/peroxidase reaction for ELISA applications and is intended for use in combination with CST's PathScan Sandwich ELISA Antibody Pairs.
Alternate Names
ELISA
(a) After incubation with horseradish peroxidase (HRP) conjugated secondary antibody, wash plates with PBS/0.05% Tween-20. (b) Add 100 μl of TMB solution/well. Incubate for a specific amount of time or until desired signal is achieved. See product datasheet for recommended incubation times for specific antibody pair. (c) Add 100 μl of STOP Solution. (d) Read at Absorbance 450 nm.
Storage
Store at 4ºC.
Background
STOP Solution is used to terminate TMB/peroxidase reaction for ELISA applications and is intended for use in combination with CST's PathScan Sandwich ELISA Antibody Pairs.
Alternate Names
ELISA
