Description
Product Usage Information
Western Blotting: 1:1000
Immunoprecipitation: 1:50
Immunofluorescence (Immunocytochemistry): 1:1600 - 1:3200
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Immunocytochemistry)
Specificity / Sensitivity
NeuroD1 (D90G12) Rabbit Monoclonal Antibody detects endogenous levels of total NeuroD1 protein.
Species Reactivity: Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly315 of human NeuroD1 protein.
Background
Neurogenic differentiation factor 1 (NeuroD1) is a member of the basic helix-loop-helix (bHLH) family of transcription factors. These proteins function by forming heterodimers with E-proteins and binding to the canonical E-box sequence CANNTG (1,2). Neuronal activity results in CaMKII-mediated phosphorylation of NeuroD1 at Ser336, which is necessary for dendrite formation and growth (3,4). NeuroD1 is also phosphorylated at Ser274, though the results are context dependent, as phosphorylation by Erk stimulates NeuroD1 activity in pancreatic β cells while phosphorylation by GSK-3β inhibits NeuroD1 in neurons (3). NeuroD1 is crucially important in both the pancreas and the developing nervous system and plays a large role in the development of the inner ear and mammalian retina (3). Mice lacking NeuroD1 become severely diabetic and die shortly after birth due to defects in β cell differentiation (2,3,5,6). The lack of NeuroD1 in the brain results in severe defects in development (5). Human mutations have been linked to a number of types of diabetes, including type I diabetes mellitus and maturity-onset diabetes of the young (1,3).
Alternate Names
basic helix-loop-helix transcription factor; beta-cell E-box transactivator 2; BETA2; BHF-1; BHLHA3; Class A basic helix-loop-helix protein 3; MODY6; NDF1; NEUROD; NEUROD1; neurogenic differentiation 1; Neurogenic differentiation factor 1; neurogenic helix-loop-helix protein NEUROD; neuronal differentiation 1; T2D
