Description
Product Usage Information
Western Blotting: 1:1000
Immunoprecipitation: 1:50
Immunofluorescence (Immunocytochemistry): 1:50 - 1:200
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting, Immunofluorescence (Immunocytochemistry)
Specificity / Sensitivity
NDUFS1 (E4K3E) Rabbit Monoclonal Antibody recognizes endogenous levels of total NDUFS1 protein.
Species Reactivity: Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ile286 of human NDUFS1 protein.
Background
NDUFS1 (NADH dehydrogenase Fe-S protein 1) is a nuclear encoded structural subunit of NADH: ubiquinone oxidoreductase (complex 1) in the mitochondrial electron transport chain (1). Mutations in NDUFS1 and other complex 1 subunits leading to mitochondrial dysfunction are associated with a number of neurological disorders (2-5). High-fat diet associated with type 2 diabetes leads to decreased expression of complex 1 subunits, including NDUFS1 (6). NDUFS1 is the target of cleavage by apoptotic caspases contributing to the loss of mitochondrial transmembrane potential, compromised mitochondrial respiration, increased mitochondrial reactive oxygen species (ROS) production, and loss of lysosomal integrity (7,8). NDUFS1 can also be cleaved by the lymphocyte protease granzyme B (9). Studies have also found that MDM2, which has a canonical role as an inhibitor of the tumor suppressor p53, can also sequester NDUFS1 in the cytoplasm, leading to respiratory dysfunction and increases in ROS (10).
Alternate Names
CI-75k; CI-75kD; complex I 75kDa subunit; Complex I-75kD; complex I, mitochondrial respiratory chain, 75-kD subunit; MC1DN5; MGC26839; mitochondrial NADH-ubiquinone oxidoreductase 75 kDa subunit; NADH dehydrogenase (ubiquinone) Fe-S protein 1, 75kDa (NADH-coenzyme Q reductase); NADH-coenzyme Q reductase; NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial; NADH:ubiquinone oxidoreductase core subunit S1; NDUFS1; NDUS1; PRO1304
