Description
Product Usage Information
Western Blotting: 1:1000
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting
Specificity / Sensitivity
Phospho-GRB2 (Tyr160) (F3B5U) Rabbit Monoclonal Antibody recognizes endogenous levels of GRB2 protein only when phosphorylated at Tyr160. This antibody may cross-reacts with other highly tyrosine phosphorylated proteins, including the HER family proteins. This antibody detects 70 kDa and 90 kDa proteins of unknown identity in some cell lines.
Species Reactivity: Human, Mouse
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr160 of human GRB2 protein.
Background
Growth factor receptor-binding protein 2 (GRB2) is an adaptor protein that is involved in RTK signal transduction. The SH2 domain of GRB2 binds to tyrosine phosphorylated proteins, such as EGFR, IRS-1, Shc, and Gab1 (1). The SH3 domain of GRB2 associates with SOS, which stimulates the Ras GTP-binding activity, leading to the activation of the MAP kinase and other signaling pathways. Phosphorylation of GRB2 at Tyr209 by Bcr-Abl and EGFR abolishes its association with SOS and negatively regulates downstream signaling (2). GRB2 is phosphorylated at Tyr160 by NPM-ALK kinase and Src kinase (3,4). The phosphorylation promotes its disassociation from dimer to monomer, stabilizes its association with SOS, and activates the downstream MAP kinase signaling (5).
Alternate Names
abundant SRC homology; Adapter protein GRB2; ASH; EGFRBP-GRB2; epidermal growth factor receptor-binding protein GRB2; epididymis secretory sperm binding protein; GRB2; Grb3-3; growth factor receptor bound protein 2; Growth factor receptor-bound protein 2; growth factor receptor-bound protein 3; HT027; MST084; MSTP084; NCKAP2; Protein Ash; SH2/SH3 adapter GRB2
