Description
Product Usage Information
Biotinylated antibodies are ideal for immunoassay technologies and high-throughput ELISA platforms that require antibody pairs where both antibodies are from the same host. Platforms utilizing biotinylated antibodies include, but are not limited to, MSD, xMAP, Quanterix Simoa, AlphaLISA, AlphaScreen, HTRF, LANCE, and TR-FRET. Optimal dilutions/working concentrations should be determined by the end user. Please contact us if you require the antibody clone biotinylated at a different concentration, a carrier-free formulation, or a more customized packaging solution.
Storage
Supplied in 140 mM NaCl, 3 mM KCl, 10 mM sodium phosphate (pH 7.4) dibasic, 2 mM potassium phosphate monobasic, 2 mg/mL BSA, and 50% glycerol. Store at -20°C. Do not aliquot the antibody.
Specificity / Sensitivity
BLK (E8T1B) Rabbit Monoclonal Antibody (Biotinylated) recognizes endogenous levels of total BLK protein.
Species Reactivity: Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro228 of human BLK protein.
Background
BLK is a Src family protein tyrosine kinase expressed in all stages of B cell development (1,2). Activation of B cells by various ligands is accompanied by activation of BLK (3). It has been suggested that BLK is involved in the control of B cell differentiation and proliferation (4,5). BLK transcripts have also been detected in human thymocytes, but not in mature T cells, implicating that BLK may play an important role in thymopoiesis (6). BLK function may be redundant, however, as mice that do not express BLK are not impaired with respect to B cell development and immune response (7).
Alternate Names
B lymphocyte kinase; B lymphoid tyrosine kinase; BLK; BLK nonreceptor tyrosine kinase; BLK proto-oncogene, Src family tyrosine kinase; MGC10442; MODY11; p55-Blk; Tyrosine-protein kinase Blk
