Description
Product Usage Information
Western Blotting: 1:1000
Simple Western™: 1:10 - 1:50
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting
Specificity / Sensitivity
Cystatin F/CST7 (F6Y9Q) Rabbit Monoclonal Antibody recognizes endogenous levels of total cystatin F/CST7 protein.
Species Reactivity: Human, Mouse
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro35 of mouse cystatin F/CST7 protein.
Background
Cystatin F, also known as CST7, is an endosomal-lysosomal cysteine protease inhibitor member of the cystatin type 2 superfamily, encoded by the gene (1). It is selectively expressed by hemopoietic cells such as cytotoxic T cells, natural killer cells, monocytes, and dendritic cells, suggesting a role in immune regulation (1,2). CST7 is expressed as an inactive dimer and converted to a functionally active monomer localized to the endo-lysosomal compartment (3). CST7 has been implicated in various cancer types, including breast and cervical cancer, and is thought to play a role in modulating the tumor microenvironment (4,5). Additionally, CST7 is highly upregulated in disease-associated microglia in Alzheimer's disease and other neurodegenerative diseases, and may play a sex-specific role in neurodegeneration (6,7).
Alternate Names
CMAP; Cst7; cystatin 7; cystatin F (leukocystatin); Cystatin-7; Cystatin-F; cystatin-like metastasis-associated protein; CYTF; leukocystatin; MGC151424; MGC151426
