Description
Product Usage Information
Western Blotting: 1:1000
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting
Specificity / Sensitivity
ACP/NDUFAB1 (E2V9B) Rabbit Monoclonal Antibody recognizes endogenous levels of total ACP/NDUFAB1 protein.
Species Reactivity: Human, Mouse, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human ACP/NDUFAB1 protein.
Background
Mitochondrial acyl carrier protein (ACP) is an essential component in fatty acid biosynthesis in mitochondria. It is also known as NADH-ubiquinone oxidoreductase subunit AB1 (NDUFAB1), a complex I subunit in the electron transport chain (ETC). NDUFAB1 regulates energy and reactive oxygen species (ROS) metabolism in mitochondria by coordinating the assembly of ETC complexes I, II, and III, and supercomplexes (1). Studies show that overexpression of this protein protects the heart from ischemia-reperfusion injury in mice as well as protects mice from insulin resistance and obesity (1,2). In addition, mRNA levels are upregulated in pancreatic ductal adenocarcinoma compared with normal pancreatic ductal tissues (3).
Alternate Names
ACP; ACP1; ACPM; Acyl carrier protein, mitochondrial; CI-SDAP; complex I SDAP subunit; FASN2A; MGC65095; mitochondrial acyl carrier protein; NADH dehydrogenase (ubiquinone) 1, alpha/beta subcomplex, 1, 8kDa; NADH-ubiquinone oxidoreductase 9.6 kDa subunit; NADH:ubiquinone oxidoreductase SDAP subunit; NADH:ubiquinone oxidoreductase subunit AB1; NDUFAB1; SDAP
