Description
Product Usage Information
Western Blotting: 1:1000
Immunofluorescence (Frozen): 1:800
Immunofluorescence (Immunocytochemistry): 1:800
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting, Immunofluorescence (Frozen), Immunofluorescence (Immunocytochemistry)
Specificity / Sensitivity
Podoplanin (F9S2C) Rabbit Monoclonal Antibody recognizes endogenous levels of total Podoplanin protein. Non-specific staining of fixed frozen mouse liver may be observed by immunofluorescence.
Species Reactivity: Mouse
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp30 of mouse podoplanin protein.
Background
Podoplanin (aggrus, glycoprotein 36) is a single-pass transmembrane protein belonging to the type-1 family of sialomucin-like glycoproteins. Podoplanin was first described in the rat as a surface glycoprotein that regulated podocyte morphology (1). It is now commonly used as a marker of lymphatic endothelial cells, where its expression is associated with the process of lymphangiogenesis (2). Its role in this regard is presumably due to its putative involvement in regulating actin cytoskeleton dynamics (3). Research studies have shown that podoplanin expression is upregulated in a number of tumor types including colorectal cancers (4), oral squamous cell carcinomas (5), and germ cell tumors (6), with higher expression levels often associated with more aggressive tumors (7). Research studies have suggested a functional role for podoplanin in the stromal microenvironment of tumors. For example, it has been reported that podoplanin expression in cancer-associated fibroblasts (CAFs) is positively associated with a stromal environment that promotes cancer progression (8,9).
Alternate Names
Aggrus; E11; Glycoprotein 38; Gp3; Gp38; OTS; OTS-8; Ots8; OTTMUSP00000010796; PA2.; PA2.26; PA2.26 antigen; Pdpn; Podoplanin; RAND; RANDAM-2; T1-alpha; T1A; T1alpha; Transmembrane glycoprotein E11
