Description
Product Usage Information
Immunohistochemistry (Paraffin): 1:25 - 1:100
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Immunohistochemistry (Paraffin)
Specificity / Sensitivity
NCR1/NKp46 (F8M2L) Rabbit Monoclonal Antibody (IHC Formulated) recognizes endogenous levels of total NCR1/NKp46 protein.
Species Reactivity: Mouse
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of mouse NCR1/NKp46 protein.
Background
The family of natural cytotoxicity receptors (NCRs) contains NCR1 (NKp46/CD335), NCR2 (NKp44/CD336), and NCR3 (NKp30/CD337). They are single-pass type I transmembrane proteins belonging to the immunoglobulin (Ig) superfamily. Various pathogen-derived and host-encoded molecules have been identified as ligands for the NCRs. They were originally identified by their ability to evoke the cytotoxicity of natural killer (NK) cells toward tumor cells , and animal models have demonstrated that NCRs play a role in tumor surveillance, viral infection, and pregnancy (1-5). NCR1/NKp46 is considered a universal marker for NK cells, and recent studies found that it is also expressed by other cells, such as group 1 innate lymphoid cells (ILC1s), a subset of group 3 ILCs (NCR+ ILC3), and γδ T cells (2-4). NCR1/NKp46 is also expressed on and considered a diagnostic marker and therapeutic target for some malignant NK, NKT, and T cell lymphomas (6,7). Cross-linking NCR1/NKp46 with antibodies can activate NK cells, which has been pursued as a promising therapeutic avenue (8).
Alternate Names
Activating receptor 1; CD335; Ly9; Ly94; Lymphocyte antigen 94; mAR-1; MAR1 (mouse activating receptor 1); mNKp46; Natural cytotoxicity triggering receptor 1; Natural killer cell p46-related protein; NCR1; NCTR1; NK-p46; NKp; NKp46
