Description
Product Usage Information
Western Blotting: 1:1000
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting
Specificity / Sensitivity
CEMIP (E4JIZ) Rabbit Monoclonal Antibody recognizes endogenous levels of total CEMIP protein.
Species Reactivity: Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human CEMIP protein.
Background
CEMIP (KIAA1199, HYBID) was originally described in patients affected by nonsyndromic hearing loss due to point mutations (1). One important role of the protein is the catabolic metabolism of hyaluronan by binding and depolymerizing the high molecular weight hyaluronan to low molecular weight hyaluronan, which contributes to cancer progression and inflammatory disease development (2,3). CEMIP further facilitates cancer progression by promoting WNT/β-catenin, EGFR, and calcium signaling pathways through direct interaction with pathway components (4-7). Aberrant high expression of CEMIP is associated with various types of cancer, where it plays key roles in tumor initiation, progression, metastasis, and epithelial-mesenchymal transition (EMT) (8). High levels of secreted CEMIP are found in inflammatory arthritis, osteoarthritis, atherosclerosis, and inflammatory bowel disease (9,10). Gene knockdown of CEMIP reduces progression of these diseases. CEMIP is a potential target for cancer and inflammatory arthritis management (11,12).
Alternate Names
CCSP1; cell migration inducing hyaluronan binding protein; cell migration inducing hyaluronidase 1; cell migration inducing protein, hyaluronan binding; Cell migration-inducing and hyaluronan-binding protein; CEMIP; colon cancer secreted protein 1; hyaluronan-binding protein involved in hyaluronan depolymerization; HYBID; KIAA1199; TMEM2L; transmembrane protein 2-like
